Problem: Current tools do not collect images and data along with tools that enable users to visualize outliers. Outliers are important because they may indicate problems in quality that require investigation.

Objective: Document and analyze images and data on cells over time to improve the quality and reproducibility of cell, tissue, and suspension cultures.

CellAssist Solution: The CellAssist Systems provide comparable data over time that allows for the identification of outliers. (In this example, upon further investigation, the customer found cases of mislabeling.)

Problem: Gene editing transfection rates can be poor, so selecting monoclonal colonies for passaging is crucial.

Objective: Accurately determine whether a colony is monoclonal by capturing a series of time-lapse images with excellent registration across scans.

CellAssist Solution: The CellAssist Systems' time-lapse imaging enables the lineage of candidate colonies to be tracked to a single starting cell.

Problem: Differentiation of neuronal progenitor cells into glial cells was measured using fluorescence-based endpoint assays, which did not allow for further growth and study of the cells. An equivalent assay was needed based on imaging of live-cells.

Objective: Acquire high-resolution images that are comparable over time to study differentiation without dyes and/or probes that interfere with the cells and colonies.

CellAssist Solution: The CellAssist Systems' high-resolution stacks of images, with highly accurate registration across scans, enabled the characterization of cell morphology without disturbing the cells and colonies.

Problem: The coating of plates can profoundly affect the survivability and growth of colonies (in this case, gene-edited ones starting with very small quantities). Some coatings are also significantly more expensive.

Objective:

1. Coating Comparison: Evaluate and compare two different coatings by observing and characterizing the behavior of cells under each condition over a set period. This analysis will help determine which coating optimally supports cell health and activity.

2. Protocol Optimization: Develop, implement, and assess new protocols tailored to these coatings. By directly comparing the results, the most effective protocol and coating combination that enhances experimental outcomes can be identified.

CellAssist Solution: The CellAssist Systems' documentation, colony counting curves, and image examination characterize the number of colonies for each condition, and the results are collated and easily compared across conditions.